Cyanine5 NHS ester
| Cat. # | Quantity | Price |
| 13020 | 1mg | 1760.00 |
| 23020 | 5mg | 3360.00 |
| 43020 | 25mg | 6560.00 |
| 53020 | 50mg | 11120.00
|
| 63020 | 100mg | 17600.00
|
During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.
This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.
Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 616.19 |
| CAS number: | 1032678-42-4, 350686-88-3 |
| Molecular formula: | C36H42ClN3O4 |
| Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
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| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
Spectral properties
在过去的一些年，Cy5荧光基因是生命科学研究和诊断领域非常受欢迎的标记试剂。在红色区域有最大的荧光发射，许多CCD检测器有最大灵敏性，并且背景低。染料颜色非常深，因此即使在凝胶上少至1nmol的量用肉眼也可观察到。
Cy5 NHS酯是用来标记多肽，蛋白和寡核苷酸氨基的活性染料。此染料只需少量的共溶剂（比如DMF或DMSO）来标记反应。此试剂用来高效标记可溶性蛋白，各种多肽和寡核苷酸的氨基。对于较复杂的靶标比如易降解的蛋白，不能用DMF或DMSO时，可以考虑用水溶性sulfo-Cyanine 5 NHS 酯不需要共溶剂，并且两者有非常相似的荧光特性。
Cy5荧光基因与各种设备兼容包括许多荧光显微镜，成像仪，扫描设备和荧光成像仪等。
Cy5 NHS酯可以替代Alexa Fluor 647, and DyLight 649和Cy3.5®活性酯。
分子结构
一般性质
外观：深蓝色粉末
分子量：616.19
分子式：C36H42ClN3O4
CAS号：1032678-42-4, 350686-88-3
溶解性：易溶于有机溶剂（DMSO,DMF和氯化物），水中的溶解性低。
质控：NMR 1H（95%）和13C，TLC, 功能测试
储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。
光谱性质：
最大激发波长：646
在最大激发波长下的消光系数：250000
最大发射波长：662
荧光量子产率：0.2
During last years, Cyanine5 flurophore (analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. Fluorophore emission has maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. Dye color is very intense, therefore quantity as small as 1 nanomol can be detected in gel electrophoresis by naked eye.
This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.
Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.
General properties
| Appearance: | dark blue powder |
| Molecular weight: | 616.19 |
| Molecular formula: | C36H42ClN3O4 |
| CAS number: | 1032678-42-4, 350686-88-3 |
| Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
|
|
Spectral properties
| Excitation maximum, nm: | 646 |
| Extinction coefficient at excitation maximum, Lmol-1cm-1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
