（备注：非常规分子量：400、600。精确聚合度的小分子定制。）

结构式：

基本信息：

中文名称：Dibenzocyclooctyne-聚乙二醇-二硬脂酰磷脂酰乙酰胺 (DBCO-PEG-DSPE)

别名：DBCO-PEG-DSPE

英文名称：DBCO-PEG-DSPE

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：90%以上

应用简介：

DBCO (dibenzocycolctyne)PEG DSPE derivatives can go Click Chemistry reaction without a need of anymetal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynesand azides, also termed as the Cu-free click reaction, is a bioorthogonalreaction that enables the conjugation of two molecules in aqueous solution.DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer.DBCO reagents can be used to label azide-modified biomolecules spontaneouswithout the need for toxic Cu catalysts. PEGylation can increase solubility andstability and reduce immunogenicity of peptides and proteins. It can alsosuppress the non-specific binding of charged molecules to the modifiedsurfaces.

Copper-freeClick reaction procedures:

Preparethe azide-containing reagents in reaction buffer.

AddDBCO reagents to azide containing reagents. Add 1 mol equivalent of limitingreagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubatethe reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.

Purifyconjugates either by dialysis or size-exclusion chromatograph.

物理性质：

状态：淡黄色到白色固体，或者半固体，取决于分子量。

溶解性：溶于水，氯仿，DMSO等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。NHS可以和NH2。

技术指标：

纯度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（DSPE）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF